Gene therapy can be adapted to each individual to treat a wide range of diseases including cancer and rare diseases. The development of suitable gene transfer vectors to introduce exogenous genes into target cells is of great importance. Currently, research in gene therapy vectors has developed from viral vectors such as retroviruses, adenoviruses, and adeno-associated viruses to today's novel vectors such as baculovirus vectors, which provide a relatively safe, scalable, and cost-effective gene transfer strategy for gene therapy. The baculovirus gene delivery system is an easily modifiable gene therapy system that has been reported to enable site-specific delivery, mitigate adverse effects and improve therapeutics, with the potential to be the cost-effective and efficient backbone for gene therapy. Although challenges remain for recombinant baculoviruses in gene therapy, their high DNA-carrying capacity, maneuverability, and ability to express multiple exogenous genes simultaneously make them promising as gene delivery systems for personalized medicine and gene therapy.