The view of scientific and diagnostic concerns may be profoundly altered by next-generation sequencing (NGS), a method. A new era of bioinformatics and biostatistical data analysis is beginning thanks to this technology's ability to generate massive volumes of sequencing data at cheaper costs. You can get the coverage you need by using Illumina sequencing technologies, which are suited for de novo sequencing at a reasonable cost. IST is renowned for its capacity to generate high-quality raw reads and to structure paired-end sequencing to provide trustworthy backbone sequences. At CBL, we use multiple read alignments to cross-reference and fix any mistakes to guarantee extremely accurate readings.