Microbial metabolites are intermediate or end products of microbial metabolism, which are involved in the various biochemical reactions in microorganisms. Microbial metabolites include various organic compounds, such as amino acids, nucleotides, fermented alcohols, signal molecules, growth factors, etc. They play an immense role in healthcare, and are a major source of essential nutrients for all life forms. The inherent characteristic of microbial metabolites is their origin, that is to say their specific producer. Different producers are related to various biological activities and functions of microbial metabolites. Microbial metabolites are classified into primary metabolites and secondary metabolites[1]. Primary metabolites Metabolites produced in early phase of microbial growth are involved in cellular growth and development and are termed as primary metabolites. Various microorganism species share similar primary metabolites, including amino acids, nucleotides, organic acids, microbial peptides, etc., which are widely used for human health. For example, microbial peptides are related in peptide vaccines and the degradation of bacterial toxins. Secondary metabolites Metabolites produced in the late phase of microbial growth and development and are termed as secondary metabolites. They are not involved in any essential functions like cell division and growth of microorganisms. Secondary metabolites include a wide range of bioactive compounds like antibiotics, toxins, enzyme inhibitors, polyketides, etc., which are widely used in various fields. For example, polyketides, a group of bioactive secondary metabolites and are used in medicine due to their diverse biological and pharmaceutical activities. Biological Activity of Secondary Metabolites Among microbial metabolites, secondary metabolites are the most widely used due to their multiple biological activities.