EnzymoGenius™ excels in delivering cutting-edge services for the rational design of focused enzyme libraries, leveraging state-of-the-art technologies to meet the evolving needs of biotechnological research. Overview The rational design of focused enzyme libraries is a strategic approach in enzyme engineering, aiming to enhance catalytic efficiency and substrate specificity. Through a meticulous analysis of enzyme structure and function, rational design leverages molecular insights to guide the creation of targeted mutant libraries. This process involves the identification of key residues and domains influencing enzymatic activity, followed by the introduction of precise mutations to modulate substrate interactions. Recent advancements in this field have seen the integration of computational tools, such as molecular dynamics simulations and structure-based algorithms, facilitating the prediction of mutant effects. By systematically optimizing enzyme properties, rational design cont

In the realm of enzymatic research, strides have been made in the design of enzymes tailored for novel substrate classes, marking a pivotal juncture in the pursuit of biocatalytic applications. Notably, recent advancements underscore the expanding scope of enzyme design, reaching beyond traditional substrates to address the catalytic challenges posed by emerging classes. Enzyme engineering strategies have harnessed computational approaches and directed evolution to fine-tune the catalytic machinery, enabling the recognition and transformation of previously untapped substrates with precision. The exploration of diverse substrate classes, ranging from synthetic compounds to naturally occurring molecules, has propelled the field toward unprecedented versatility. Furthermore, the integration of structural bioinformatics has played a pivotal role in deciphering the intricate interactions governing substrate specificity. As researchers delve into uncharted enzymatic territories, the converge

One of the key components of synthetic biology is the creation of novel strains with unique properties that can be used for various applications such as biotechnology, medicine, and agriculture. Strain hybridization is an essential technique in synthetic biology that involves the combination of genetic material from different strains to create a new hybrid strain with desired traits.

CRISPR (clustered regularly interspaced short palindromic repeat) is a segment of nucleic acid containing short, repetitive base sequences derived from DNA fragments of bacteriophages that had previously infected prokaryotic cells. These sequences, found in prokaryotic genomes, play an important role in prokaryotic defense. The prokaryotic immune system consisting of CRISPR and Cas (CRISPR-associated) proteins now form the basis of genome editing technology. CRISPR-mediated genome editing is a rapidly expanding field. In addition to its widespread use in basic biology research such as gene knockdown or knock-in, gene repression or activation, genomic DNA tagging, and gene screening, the CRISPR-Cas system is also a powerful tool for the development of biological products and therapeutics. The core concept of synthetic biology is to construct artificial decision-making circuits by building and integrating standardized components and modules. The early stage of synthetic biology mainly

Cell-free protein expression or cell-free protein synthesis (CFPS) refers to the protein expression approach that produces target proteins without using living cells. All the cellular machinery, such as ribosomes, enzymes, tRNAs, amino acids, and cofactors, are contained in CFPS for direct protein synthesis. A supplied nucleic acid template can be transcribed and translated in CFPS, and this process is not constrained by a cell wall or homeostasis conditions required to maintain cell viability.

The functions of biological macromolecules mainly depend on their three-dimensional structure, movements, and interactions. Protein structure analysis can provide information on protein classification, protein function, protein interactions, posttranslational modifications, and many other aspects. It fundamentally clarifies the molecular mechanism, and is also an important way to study protein function. At present, there are three main methods to determine the 3D structure of proteins, including X-ray crystallography, nuclear magnetic resonance technology (NMR), and 3D reconstruction technology of cryo-electron microscopy. One of the central goals of synthetic structural biology is to create proteins with novel structures. Therefore, protein structure determination plays a critical role in synthetic biology.

Proteins are complex molecular entities with different sizes, molecular structures, and physical and chemical properties. Protein characterization is one of the important parts of protein expression. Characterizing a protein can obtain useful information on its identity, purity, structure, conformation, and activity. The precise molecular weight, amino acid sequence, charge variances, aggregation level, glycosylation, and oxidation level are the key to the in-depth characterization of proteins. Different post-translational modifications, highly specific three-dimensional structures, and the potential for aggregation, adsorption, and truncation determine the complexity of proteins. No one analytical platform or technique can meet all the requirements to characterize proteins so far.

Site-directed mutagenesis is a very useful in vitro technique to create specific, targeted mutations in a known DNA sequence. It can efficiently change the characterization of target proteins. Site-directed mutagenesis can be used as a precision tool to enable synthetic biology. It plays an essential role in a variety of synthetic biology research, such as the study of gene regulatory elements, protein structure and functions, enzyme active sites and novel proteins.

As a fast-growing and innovative company, CD Biosynsis has established a powerful synthetic biology platform to integrate engineering principles and a broad range of methodologies from various disciplines, including biology, bioinformatics, chemistry, physics, mathematics, and computer science. We have built a multidisciplinary team dedicated to developing novel synthetic biology tools and incorporating emerging technologies into our platform, giving us the ability to provide custom synthetic biology services and effective strategies to accelerate our customers’ synthetic biology research and development processes.

Site-directed mutagenesis is a very useful in vitro technique to create specific, targeted mutations in a known DNA sequence. It can efficiently change the characterization of target proteins. Site-directed mutagenesis can be used as a precision tool to enable synthetic biology. It plays an essential role in a variety of synthetic biology research, such as the study of gene regulatory elements, protein structure and functions, enzyme active sites and novel proteins.

The functions of biological macromolecules mainly depend on their three-dimensional structure, movements, and interactions. Protein structure analysis can provide information on protein classification, protein function, protein interactions, posttranslational modifications, and many other aspects. It fundamentally clarifies the molecular mechanism, and is also an important way to study protein function. At present, there are three main methods to determine the 3D structure of proteins, including X-ray crystallography, nuclear magnetic resonance technology (NMR), and 3D reconstruction technology of cryo-electron microscopy. One of the central goals of synthetic structural biology is to create proteins with novel structures. Therefore, protein structure determination plays a critical role in synthetic biology.

The Synthetic Biology Platform is a comprehensive toolbox and fully integrated platform to empower synthetic biology research and development. The Synthetic Biology Platform is a comprehensive toolbox and fully integrated platform to empower synthetic biology research and development. Synthetic biology is an emerging interdisciplinary field that combines principles of engineering and biology to create artificial lives or re-design organisms for useful purposes. In fact, designing a platform that could carry out full-scale biosynthesis development from chemicals to enzymes in one place can save much cost and time for synthetic biology researchers. Given the need, a cutting-edge Synthetic Biology Platform is developed to offer technical support for the development of synthetic biology applications. The Synthetic Biology Platform integrated with the CRISPR technology can regulate gene expression needed for synthetic biology more efficiently and precisely. Complex genetic circuit

The Synthetic Biology Platform is designed to program comprehensive biosynthesis development in one place. The goal was to develop a platform that could program comprehensive biosynthesis development in one place, and since then, it has become a reality. The Synthetic Biology Platform is a disruptive technology platform focusing on synthetic biology, which can contribute to the advancement of diverse areas, including but not limited to industrial biotechnology, pharmaceutical, agriculture, and healthcare research. The Synthetic Biology Platform integrates engineering principles and a broad range of methodologies from various disciplines, such as biology, bioinformatics, chemistry, physics, mathematics, and computer science. It’s designed to perform strain-based chassis engineering as well as biosynthesis development for various molecules, thus gaining insights into disease mechanisms, identifying novel drug targets, and accelerating drug discovery and development. Combining th