Plant cell walls are complex 3D networks composed of proteins, polysaccharides, and lignin. Multiple molecular interactions make the analysis of cell wall compounds a more complex task. In order to analyze ex-situ cell wall components, their destructuration /dissociation/disorganization from each other is a prerequisite for their analysis. The dissociation of plant cell walls depends heavily on the number and type of bonds (covalent, electrostatic, hydrogen, ionic linkage) between them. The disintegration of bonds between these polymers can be achieved by chemical, physical, enzymatic, or by a mixture of two or three of these methods. Developing efficient and rapid methods to release all cell wall components without altering their chemical compositions is currently a major challenge in the in vitro analysis of cell wall components.